cav3 1 n terminal rabbit polyclonal alomone  (Alomone Labs)

Journal: Neural Regeneration Research

Article Title: L- and T-type Ca 2+ channels dichotomously contribute to retinal ganglion cell injury in experimental glaucoma

doi: 10.4103/1673-5374.360277

Figure Lengend Snippet: Changes in protein levels of L- and T-type Ca 2+ channel subunits in COH retinas. (A) Representative immunoblots showing the expressions of Ca V 1.2 (a L-type Ca 2+ channel subunit) and Ca V 3.1, Ca v 3.2, and Ca V 3.3 (T-type Ca 2+ channel subunits) in sham-operated (control) and COH retinal extracts at the indicated post-operational times. (B–E) Bar charts showing the densitometric quantification of immunoreactive bands of Ca v 1.2 (B), Ca v 3.1 (C), CaV3.2 (D), and Ca V 3.3 (E) under the conditions as shown in A. (F) Representative immunoblots showing the expression of Brn3a, a RGC marker, in control and COH retinal extracts at the indicated post-operational times. (G, H) Bar charts showing Brn3a protein levels (G) and Ca v 1.2/Brn3a ratios (H) under the conditions as shown in A and F. Data are presented as the mean ± SEM ( n = 6 for all groups). * P < 0.05, ** P < 0.01, *** P < 0.001, vs . control (one-way analysis of variance test with Bonferroni’s post hoc test (for B–D, G and H) and non-parametric Kruskal-Wallis test (for E)). COH: Chronic ocular hypertension; RGC: retinal ganglion cell.

Article Snippet: The primary antibodies used in this study were mouse anti-Ca V 1.2 monoclonal antibody (1:50, Abcam, Cambridge, MA, USA, Cat# ab84814, RRID: AB_1860052), rabbit anti-Ca V 3.1 polyclonal antibody (1:100, Alomone, Jerusalem, Israel, Cat# ACC-021, RRID: AB_2039779), rabbit anti-Ca V 3.2 polyclonal antibody (1:100, Alomone, Cat# ACC-025, RRID: AB_2039781), rabbit anti-Ca V 3.3 polyclonal antibody (1:100, Alomone, Cat# ACC-009, RRID: AB_2039783), goat anti-Brn-3a polyclonal antibody (1:300, Santa Cruz Biotechnology, Santa Cruz, TX, USA, Cat# sc-31984, RRID: AB_2167511), and mouse anti-β-actin (1:10,000, Sigma-Aldrich, Cat# A5316, RRID: AB_476743).

Techniques: Western Blot, Control, Expressing, Marker, Analysis

Journal: Neural Regeneration Research

Article Title: L- and T-type Ca 2+ channels dichotomously contribute to retinal ganglion cell injury in experimental glaucoma

doi: 10.4103/1673-5374.360277

Figure Lengend Snippet: Changes in protein levels of L- and T-type Ca 2+ channel subunits in COH retinas. (A) Representative immunoblots showing the expressions of Ca V 1.2 (a L-type Ca 2+ channel subunit) and Ca V 3.1, Ca v 3.2, and Ca V 3.3 (T-type Ca 2+ channel subunits) in sham-operated (control) and COH retinal extracts at the indicated post-operational times. (B–E) Bar charts showing the densitometric quantification of immunoreactive bands of Ca v 1.2 (B), Ca v 3.1 (C), CaV3.2 (D), and Ca V 3.3 (E) under the conditions as shown in A. (F) Representative immunoblots showing the expression of Brn3a, a RGC marker, in control and COH retinal extracts at the indicated post-operational times. (G, H) Bar charts showing Brn3a protein levels (G) and Ca v 1.2/Brn3a ratios (H) under the conditions as shown in A and F. Data are presented as the mean ± SEM ( n = 6 for all groups). * P < 0.05, ** P < 0.01, *** P < 0.001, vs . control (one-way analysis of variance test with Bonferroni’s post hoc test (for B–D, G and H) and non-parametric Kruskal-Wallis test (for E)). COH: Chronic ocular hypertension; RGC: retinal ganglion cell.

Article Snippet: The sections were incubated with primary antibodies: goat-anti-Brn-3a monoclonal antibody (1:400, Santa Cruz Biotechnology, Cat# sc-31984, RRID: AB_2167511), mouse-anti-Ca V 1.2 monoclonal antibody (1:100, Abcam, Cat# ab84814, RRID: AB_1860052), rabbit-anti-Ca V 3.1 polyclonal antibody (1:100, Alomone, Cat# ACC-021, RRID: AB_2039779), rabbit-anti-Ca V 3.2 polyclonal antibody (1:100, Alomone, Cat# ACC-025, RRID: AB_2039781), and rabbit-anti-Ca V 3.3 polyclonal antibody (1:100, Alomone, Cat# ACC-009, RRID: AB_2039783) at 4°C for 48 hours.

Techniques: Western Blot, Control, Expressing, Marker, Analysis

Journal: Neural Regeneration Research

Article Title: L- and T-type Ca 2+ channels dichotomously contribute to retinal ganglion cell injury in experimental glaucoma

doi: 10.4103/1673-5374.360277

Figure Lengend Snippet: TNF-α induces changes in protein levels of Ca V 1.2 and Ca V 3.3 in retinas of control rats. (A) Representative western blot results displaying the TNF-α-induced alterations of L-type Ca 2+ channel subunit Ca V 1.2. Co-injection of the TNF-α blocker XPro1595 reversed the effects of TNF-α. (B) Bar charts showing the densitometric quantification of immunoreactive bands of Ca V 1.2 under different conditions as shown in A. (C) Representative immunoblots showing the TNF-α-induced changes in the expression of T-type Ca 2+ channel subunit CaV3.3. Co-injection of the TNF-α blocker XPro1595 reversed the effects of TNF-α. (D) Bar charts showing densitometric quantification of immunoreactive bands of Ca V 3.3 under different conditions as shown in C. Data are presented as the mean ± SEM ( n = 6–7). * P < 0.05, vs . control (one-way analysis of variance test with Bonferroni’s post hoc test). TNF-α: Tumor necrosis factor-α.

Article Snippet: The sections were incubated with primary antibodies: goat-anti-Brn-3a monoclonal antibody (1:400, Santa Cruz Biotechnology, Cat# sc-31984, RRID: AB_2167511), mouse-anti-Ca V 1.2 monoclonal antibody (1:100, Abcam, Cat# ab84814, RRID: AB_1860052), rabbit-anti-Ca V 3.1 polyclonal antibody (1:100, Alomone, Cat# ACC-021, RRID: AB_2039779), rabbit-anti-Ca V 3.2 polyclonal antibody (1:100, Alomone, Cat# ACC-025, RRID: AB_2039781), and rabbit-anti-Ca V 3.3 polyclonal antibody (1:100, Alomone, Cat# ACC-009, RRID: AB_2039783) at 4°C for 48 hours.

Techniques: Control, Western Blot, Injection, Expressing, Analysis